SkQ1 accelerates dermal wound healing in animals
To Homo sapiens liberatus et invulnerabilis

I.A. Demianenko1,3, T.V. Vasilieva1,3
L.V. Domnina2,3, V.B. Dugina2,3, M.V. Egorov3,
O.Y. Ivanova2,3, O.P. Ilinskaya1,3,
O.Y. Pletjushkina2,3, E.N. Popova2,3,
I.Y. Sakharov4, A.V. Fedorov1,3,
B.V. Chernyak2,3*
1Biological Faculty, Moscow State University, Moscow 119991
2A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University
3Institute of Mitoengineering, Moscow State University
4Chemical Faculty, Moscow State University

It was shown that the novel mitochondria-targeted antioxidant SkQ1, (10-(6'-plastoquinonyl) decyltriphenylphosphonium) stimulated healing of full-thickness dermal wounds in mice and rats. Treatment with nanomolar doses of SkQ1 in various formulations accelerated wound cleaning and suppressed neutrophil infiltration at the early (7 h) steps of inflammatory phase. The effect was observed when rats were daily injected subcutaneously around the wound with 200 nM SkQ1 solution (0.05 nmol/kg) or the wounds were covered with the synthetic fil contained SkQ1 (0.019 μg/g). Local treatment with SkQ1 stimulated formation of granulation tissue and increased the content of myofibroblasts in the beginning of regenerative phase of wound healing. At the later steps SkQ1 accelerated accumulation of collagen fibers produced by myofibroblasts and re-epithelization of the wound. Lifelong treatment of mice with 30 nmol SkQ1/kg per day supplemented with drinking water strongly stimulated wound healing in old (28 months) animals. The similar effect was observed if SkQ1 was supplied only during the last 4 months. In the in vitro model of wound in human cell cultures SkQ1 stimulated movement of epitheliocytes and fibroblasts into the "wound". Myofibroblast differentiation of subcutaneous fibroblasts was stimulated by SkQ1. It is suggested that SkQ1 stimulated wound healing by suppression of the negative effects of oxidative stress in the wound and also by induction of the cell differentiation.

Homo Sapiens Liberatus Workshop, Moscow State University, May 2010